Fig. 4. A. Effect of extracellular calcium overload on the percentage of annexin V binding erythrocytes. Original histogram and arithmetic means ± SEM (n = 10) of annexin V binding of erythrocytes following exposure to Ringer solution without (black line and white bar) or with (red line and black bar) the presence of 1 µM ionomycin or with solvent alone (striped grey bar). B. Effect of extracellular calcium overload on erythrocyte forward scatter. Original histogram and arithmetic means ± SEM (n = 10) of forward scatter of erythrocytes following exposure to Ringer solution without (black line and white bar) and with (red line and black bar) presence of 1 µM ionomycin or with solvent alone (striped grey bar). ****(p<0.001) indicates significant difference from the absence of ionomycin (ANOVA). C. Effect of extracellular calcium overload on cytosolic Ca²⁺ concentration. Original histogram and arithmetic means ± SEM (n = 10) of Fluo3 fluorescence reflecting cytosolic Ca²⁺ concentration in erythrocytes following exposure to Ringer solution without (black line and white bar) and with (red line and black bar) presence of 1 µM ionomycin or with solvent alone (striped grey bar). ****(p<0.001) indicates significant difference from the absence of ionomycin (ANOVA).